Validation of dental X-ray by cytokine RANTES – comparison of X-ray findings with cytokine overexpression in jawbone
Identifieur interne : 002A93 ( Pmc/Curation ); précédent : 002A92; suivant : 002A94Validation of dental X-ray by cytokine RANTES – comparison of X-ray findings with cytokine overexpression in jawbone
Auteurs : Johann Lechner [Allemagne]Source :
- Clinical, Cosmetic and Investigational Dentistry [ 1179-1357 ] ; 2014.
Abstract
There is a need to clarify the extent to which the most common diagnostic tool in dentistry – two-dimensional panoramic tomography (2D-OPG) – is suitable for identifying fatty degenerative osteolysis of jawbone (FDOJ).
To obtain a qualitative assessment of edentulous jawbone sections, the results from 2D-OPG with a defined X-ray density (XrDn), expression of the cytokine RANTES (regulated on activation, normal T-cell expressed and secreted), and a transalveolar ultrasound system for measuring jawbone density were compared.
The difference in the XrDn of healthy jawbone and FDOJ are minimal, whereas RANTES is up to 25-fold higher in FDOJ. In contrast to 2D-OPG, transalveolar ultrasound showed coincidental findings in FDOJ areas.
Comparisons of the data revealed a discrepancy between the XrDn of 2D-OPGs and the medullary osteopathies in the jawbone like FDOJ.
The data suggest that there is a critical attitude toward the use of 2D-OPG as a sole imaging diagnostic tool for assessing chronic inflammatory processes in the jawbone. Specifically, 2D-OPG is objectively not suitable for depicting FDOJ.
Url:
DOI: 10.2147/CCIDE.S69807
PubMed: 25170282
PubMed Central: 4144985
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Validation of dental X-ray by cytokine RANTES – comparison of X-ray findings with cytokine overexpression in jawbone</title><author><name sortKey="Lechner, Johann" sort="Lechner, Johann" uniqKey="Lechner J" first="Johann" last="Lechner">Johann Lechner</name><affiliation wicri:level="1"><nlm:aff id="af1-ccide-6-071">Clinic for Integrative Dentistry, Munich, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Clinic for Integrative Dentistry, Munich</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25170282</idno><idno type="pmc">4144985</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4144985</idno><idno type="RBID">PMC:4144985</idno><idno type="doi">10.2147/CCIDE.S69807</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">002A93</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002A93</idno><idno type="wicri:Area/Pmc/Curation">002A93</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">002A93</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Validation of dental X-ray by cytokine RANTES – comparison of X-ray findings with cytokine overexpression in jawbone</title><author><name sortKey="Lechner, Johann" sort="Lechner, Johann" uniqKey="Lechner J" first="Johann" last="Lechner">Johann Lechner</name><affiliation wicri:level="1"><nlm:aff id="af1-ccide-6-071">Clinic for Integrative Dentistry, Munich, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Clinic for Integrative Dentistry, Munich</wicri:regionArea></affiliation></author></analytic><series><title level="j">Clinical, Cosmetic and Investigational Dentistry</title><idno type="eISSN">1179-1357</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Introduction</title><p>There is a need to clarify the extent to which the most common diagnostic tool in dentistry – two-dimensional panoramic tomography (2D-OPG) – is suitable for identifying fatty degenerative osteolysis of jawbone (FDOJ).</p></sec><sec><title>Materials and methods</title><p>To obtain a qualitative assessment of edentulous jawbone sections, the results from 2D-OPG with a defined X-ray density (XrDn), expression of the cytokine RANTES (regulated on activation, normal T-cell expressed and secreted), and a transalveolar ultrasound system for measuring jawbone density were compared.</p></sec><sec><title>Results</title><p>The difference in the XrDn of healthy jawbone and FDOJ are minimal, whereas RANTES is up to 25-fold higher in FDOJ. In contrast to 2D-OPG, transalveolar ultrasound showed coincidental findings in FDOJ areas.</p></sec><sec><title>Discussion</title><p>Comparisons of the data revealed a discrepancy between the XrDn of 2D-OPGs and the medullary osteopathies in the jawbone like FDOJ.</p></sec><sec><title>Conclusion</title><p>The data suggest that there is a critical attitude toward the use of 2D-OPG as a sole imaging diagnostic tool for assessing chronic inflammatory processes in the jawbone. Specifically, 2D-OPG is objectively not suitable for depicting FDOJ.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Patel, S" uniqKey="Patel S">S Patel</name></author><author><name sortKey="Dawood, A" uniqKey="Dawood A">A Dawood</name></author><author><name sortKey="Mannocci, F" uniqKey="Mannocci F">F Mannocci</name></author><author><name sortKey="Wilson, R" uniqKey="Wilson R">R Wilson</name></author><author><name sortKey="Pitt Ford, T" uniqKey="Pitt Ford T">T Pitt Ford</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Al Nawas, B" uniqKey="Al Nawas B">B Al-Nawas</name></author><author><name sortKey="Klein, Mo" uniqKey="Klein M">MO Klein</name></author><author><name sortKey="Gotz, H" uniqKey="Gotz H">H Götz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schwartz, Sf" uniqKey="Schwartz S">SF Schwartz</name></author><author><name sortKey="Foster, Jk" uniqKey="Foster J">JK Foster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ardran, Gm" uniqKey="Ardran G">GM Ardran</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bender, Ib" uniqKey="Bender I">IB Bender</name></author><author><name sortKey="Seltzer, S" uniqKey="Seltzer S">S Seltzer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lechner, J" uniqKey="Lechner J">J Lechner</name></author><author><name sortKey="Von Baehr, V" uniqKey="Von Baehr V">V von Baehr</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dotti, R" uniqKey="Dotti R">R Dotti</name></author><author><name sortKey="Muller, Dm" uniqKey="Muller D">DM Müller</name></author><author><name sortKey="Benini, A" uniqKey="Benini A">A Benini</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Segall, Ro" uniqKey="Segall R">RO Segall</name></author><author><name sortKey="Del Rio, Ce" uniqKey="Del Rio C">CE del Rio</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bouquot, J" uniqKey="Bouquot J">J Bouquot</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bouquot, Je" uniqKey="Bouquot J">JE Bouquot</name></author><author><name sortKey="Shankland, We" uniqKey="Shankland W">WE Shankland</name></author><author><name sortKey="Margolis, M" uniqKey="Margolis M">M Margolis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shankland, We" uniqKey="Shankland W">WE Shankland</name></author><author><name sortKey="Bouquot, Je" uniqKey="Bouquot J">JE Bouquot</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Levy, Ja" uniqKey="Levy J">JA Levy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Appay, V" uniqKey="Appay V">V Appay</name></author><author><name sortKey="Rowland Jones, Sl" uniqKey="Rowland Jones S">SL Rowland-Jones</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Von Luettichau, I" uniqKey="Von Luettichau I">I von Luettichau</name></author><author><name sortKey="Nelson, Pj" uniqKey="Nelson P">PJ Nelson</name></author><author><name sortKey="Pattison, Jm" uniqKey="Pattison J">JM Pattison</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rathanaswami, P" uniqKey="Rathanaswami P">P Rathanaswami</name></author><author><name sortKey="Hachicha, M" uniqKey="Hachicha M">M Hachicha</name></author><author><name sortKey="Sadick, M" uniqKey="Sadick M">M Sadick</name></author><author><name sortKey="Schall, Tj" uniqKey="Schall T">TJ Schall</name></author><author><name sortKey="Mccoll, Sr" uniqKey="Mccoll S">SR McColl</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bolin, Lm" uniqKey="Bolin L">LM Bolin</name></author><author><name sortKey="Murray, R" uniqKey="Murray R">R Murray</name></author><author><name sortKey="Lukacs, Nw" uniqKey="Lukacs N">NW Lukacs</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fischer, M" uniqKey="Fischer M">M Fischer</name></author><author><name sortKey="Juremalm, M" uniqKey="Juremalm M">M Juremalm</name></author><author><name sortKey="Olsson, N" uniqKey="Olsson N">N Olsson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Soria, G" uniqKey="Soria G">G Soria</name></author><author><name sortKey="Ben Baruch, A" uniqKey="Ben Baruch A">A Ben-Baruch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Niwa, Y" uniqKey="Niwa Y">Y Niwa</name></author><author><name sortKey="Akamatsu, H" uniqKey="Akamatsu H">H Akamatsu</name></author><author><name sortKey="Niwa, H" uniqKey="Niwa H">H Niwa</name></author><author><name sortKey="Sumi, H" uniqKey="Sumi H">H Sumi</name></author><author><name sortKey="Ozaki, Y" uniqKey="Ozaki Y">Y Ozaki</name></author><author><name sortKey="Abe, A" uniqKey="Abe A">A Abe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Azenshtein, E" uniqKey="Azenshtein E">E Azenshtein</name></author><author><name sortKey="Luboshits, G" uniqKey="Luboshits G">G Luboshits</name></author><author><name sortKey="Shina, S" uniqKey="Shina S">S Shina</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bouquot, J" uniqKey="Bouquot J">J Bouquot</name></author><author><name sortKey="Martin, W" uniqKey="Martin W">W Martin</name></author><author><name sortKey="Wrobleski, G" uniqKey="Wrobleski G">G Wrobleski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bouquot, Je" uniqKey="Bouquot J">JE Bouquot</name></author><author><name sortKey="Shankland, We" uniqKey="Shankland W">WE Shankland</name></author><author><name sortKey="Margolis, M" uniqKey="Margolis M">M Margolis</name></author><author><name sortKey="Glaros, W" uniqKey="Glaros W">W Glaros</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Clin Cosmet Investig Dent</journal-id><journal-id journal-id-type="iso-abbrev">Clin Cosmet Investig Dent</journal-id><journal-id journal-id-type="publisher-id">Clinical, Cosmetic and Investigational Dentistry</journal-id><journal-title-group><journal-title>Clinical, Cosmetic and Investigational Dentistry</journal-title></journal-title-group><issn pub-type="epub">1179-1357</issn><publisher><publisher-name>Dove Medical Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25170282</article-id><article-id pub-id-type="pmc">4144985</article-id><article-id pub-id-type="doi">10.2147/CCIDE.S69807</article-id><article-id pub-id-type="publisher-id">ccide-6-071</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Research</subject></subj-group></article-categories><title-group><article-title>Validation of dental X-ray by cytokine RANTES – comparison of X-ray findings with cytokine overexpression in jawbone</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Lechner</surname><given-names>Johann</given-names></name><xref ref-type="aff" rid="af1-ccide-6-071"></xref><xref ref-type="corresp" rid="c1-ccide-6-071"></xref></contrib><aff id="af1-ccide-6-071">Clinic for Integrative Dentistry, Munich, Germany</aff></contrib-group><author-notes><corresp id="c1-ccide-6-071">Correspondence: Johann Lechner, Clinic for Complementary Dentistry, 10A Gruenwalder St, 81547 Munich, Germany, Tel +49 89 697 0055, Fax +49 89 692 5830, Email <email>drlechner@aol.com</email></corresp></author-notes><pub-date pub-type="collection"><year>2014</year></pub-date><pub-date pub-type="epub"><day>21</day><month>8</month><year>2014</year></pub-date><volume>6</volume><fpage>71</fpage><lpage>79</lpage><permissions><copyright-statement>© 2014 Lechner. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License</copyright-statement><copyright-year>2014</copyright-year><license><license-p>The full terms of the License are available at <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc/3.0/">http://creativecommons.org/licenses/by-nc/3.0/</ext-link>. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.</license-p></license></permissions><abstract><sec><title>Introduction</title><p>There is a need to clarify the extent to which the most common diagnostic tool in dentistry – two-dimensional panoramic tomography (2D-OPG) – is suitable for identifying fatty degenerative osteolysis of jawbone (FDOJ).</p></sec><sec><title>Materials and methods</title><p>To obtain a qualitative assessment of edentulous jawbone sections, the results from 2D-OPG with a defined X-ray density (XrDn), expression of the cytokine RANTES (regulated on activation, normal T-cell expressed and secreted), and a transalveolar ultrasound system for measuring jawbone density were compared.</p></sec><sec><title>Results</title><p>The difference in the XrDn of healthy jawbone and FDOJ are minimal, whereas RANTES is up to 25-fold higher in FDOJ. In contrast to 2D-OPG, transalveolar ultrasound showed coincidental findings in FDOJ areas.</p></sec><sec><title>Discussion</title><p>Comparisons of the data revealed a discrepancy between the XrDn of 2D-OPGs and the medullary osteopathies in the jawbone like FDOJ.</p></sec><sec><title>Conclusion</title><p>The data suggest that there is a critical attitude toward the use of 2D-OPG as a sole imaging diagnostic tool for assessing chronic inflammatory processes in the jawbone. Specifically, 2D-OPG is objectively not suitable for depicting FDOJ.</p></sec></abstract><kwd-group><title>Keywords</title><kwd>osteonecrotic jawbone</kwd><kwd>silent inflammation</kwd><kwd>RANTES</kwd><kwd>bone density measurement</kwd></kwd-group></article-meta></front><floats-group><fig id="f1-ccide-6-071" position="float"><label>Figure 1</label><caption><p>Fatty degenerative osteolysis/osteonecrosis with typical fatty degenerative changes.</p></caption><graphic xlink:href="ccide-6-071Fig1"></graphic></fig><fig id="f2-ccide-6-071" position="float"><label>Figure 2</label><caption><p>Principle of transalveolar ultrasound measurement of bone density.</p><p><bold>Note:</bold> Explanation of grading: green = hard matter, Grade 1; yellow = ischemic process, preliminary stage, Grade 2; orange = osteonecrotic area, Grade 3; Red = osteonecrosis/osteolysis, Grade 4.</p></caption><graphic xlink:href="ccide-6-071Fig2"></graphic></fig><fig id="f3-ccide-6-071" position="float"><label>Figure 3</label><caption><p>Procedure for measuring X-ray density in four defined areas.</p><p><bold>Note:</bold> Numbers in each box describe X-ray density without units according to Kodak Dental Imaging software version 6.12.11.0 (Eastman Kodak, Rochester, NY, USA).</p></caption><graphic xlink:href="ccide-6-071Fig3"></graphic></fig><fig id="f4-ccide-6-071" position="float"><label>Figure 4</label><caption><p>Distribution of seven cytokines and X-ray density in healthy controls (n=19).</p><p><bold>Abbreviations:</bold> FGF-2, fibroblast growth factor 2; IL-1ra, interleukin 1 receptor antagonist; IL, interleukin; MCP-1, monocyte chemoattractant protein 1; TNFα, tumor necrosis factor alpha; RANTES, regulated upon activation, normal T-cell expressed and secreted chemokine (C-C motif) ligand 5; XrDn norm, X-ray density in the group of healthy jawbone; n, number; Ctrl cytokines, cytokine expression in the control group; Ctrl XrDn mean, mean values of the X-ray density in healthy jawbone.</p></caption><graphic xlink:href="ccide-6-071Fig4"></graphic></fig><fig id="f5-ccide-6-071" position="float"><label>Figure 5</label><caption><p>Distribution of seven cytokines in 31 FDOJ samples and 19 healthy controls.</p><p><bold>Note:</bold> The right green column is the mean XrDn in FDOJ cases (n=31).</p><p><bold>Abbreviations:</bold> FGF-2, fibroblast growth factor 2; IL-1ra, interleukin 1 receptor antagonist; IL, interleukin; MCP-1, monocyte chemoattractant protein 1; TNFα, tumor necrosis factor alpha; RANTES, regulated upon activation, normal T-cell expressed and secreted chemokine (C-C motif) ligand 5; XrDn FDOJ preop, X-ray density in areas of fatty degenerative osteonecrosis of jawbone before operation; n, number; FDOJ, fatty degenerative osteolysis of jawbone; Ctrl, control; XrDn, X-ray density.</p></caption><graphic xlink:href="ccide-6-071Fig5"></graphic></fig><fig id="f6-ccide-6-071" position="float"><label>Figure 6</label><caption><p>Comparison of RANTES expression and TAU in FDOJ (n=31) and healthy controls (n=19).</p><p><bold>Abbreviations:</bold> RANTES, regulated upon activation, normal T-cell expressed and secreted chemokine (C-C motif) ligand 5; TAU, transalveolar ultrasonography; FDOJ, fatty degenerative osteonecrosis of jawbone; n, number; Ctrl, control.</p></caption><graphic xlink:href="ccide-6-071Fig6"></graphic></fig><fig id="f7-ccide-6-071" position="float"><label>Figure 7</label><caption><p>Distribution of RANTES in 31 FDOJ cases and 19 samples of normal jawbone in comparison to XrDn for both groups in the corresponding areas.</p><p><bold>Abbreviations:</bold> RANTES, regulated upon activation, normal T-cell expressed and secreted chemokine (C-C motif) ligand 5; XrDn, X-ray density; FDOJ, fatty degenerative osteonecrosis of jawbone; n, number; Ctrl, control.</p></caption><graphic xlink:href="ccide-6-071Fig7"></graphic></fig><fig id="f8-ccide-6-071" position="float"><label>Figure 8</label><caption><p>Intraoperative documentation of the extension of FDOJ (<bold>B</bold>) in the right lower jawbone, area 48/49, by contrast agent (arrow) in contrast to normal XrDn (<bold>A</bold>).</p><p><bold>Notes:</bold> Red column is mean preoperative XrDn in 31 FDOJ cases; blue column is XrDn in 15 normal jawbone cases; green column is XrDn in this specific FDOJ area 48/49 before operation, although contrast medium documents huge FDOJ cavitational extent.</p><p><bold>Abbreviations:</bold> XrDn, X-ray density; FDOJ, fatty degenerative osteolysis of jawbone; n, number.</p></caption><graphic xlink:href="ccide-6-071Fig8"></graphic></fig><fig id="f9-ccide-6-071" position="float"><label>Figure 9</label><caption><p>Comparison of 2D-OPG and the average preoperative and postoperative X-ray densities for area 38/39.</p><p><bold>Notes:</bold> (<bold>A</bold>) X-ray density area 38/39; preoperative =140. (<bold>B</bold>) X-ray density area 38/39; postoperative =138. Red circles indicate retromolar areas of X-ray diagnosis before and after surgical debridement of FDOJ.</p><p><bold>Abbreviation:</bold> 2D-OPG, two-dimensional panoramic tomography.</p></caption><graphic xlink:href="ccide-6-071Fig9"></graphic></fig><fig id="f10-ccide-6-071" position="float"><label>Figure 10</label><caption><p>Seven cytokines in FDOJ in region 38/39 in comparison with control and preop and postop XrDn.</p><p><bold>Abbreviations:</bold> FGF-2, fibroblast growth factor 2; IL-1ra, interleukin 1 receptor antagonist; IL, interleukin; MCP-1, monocyte chemoattractant protein 1; TNFα, tumor necrosis factor alpha; RANTES, regulated upon activation, normal T-cell expressed and secreted chemokine (C-C motif) ligand 5; Pat LN, patient LN; Ctrl, control; n, number; preop XrDn, X-ray density before the operation; postop XrDn, X-ray density after the operation; FDOJ, fatty degenerative osteolysis of jawbone; XrDn, X-ray density.</p></caption><graphic xlink:href="ccide-6-071Fig10"></graphic></fig><fig id="f11-ccide-6-071" position="float"><label>Figure 11</label><caption><p>Comparison of XrDn in healthy Ctrl (n=19), XrDn preop and postop with RANTES (pg/mL) in FDOJ (n=15), and in normal jawbone (n=19).</p><p><bold>Abbreviations:</bold> XrDn Ctrl, X-ray density control group; n, number; XrDn preop, X-ray density before the operation; XrDn postop, X-ray density after the operation; RANTES, regulated upon activation, normal T-cell expressed and secreted chemokine (C-C motif) ligand 5; TAU grade preop, transalveolar ultrasonography before the operation; Ctrl, control; FDOJ, fatty degenerative osteonecrosis of jawbone; FDOJ XrDn, X-ray density in areas of fatty degenerative osteonecrosis of jawbone; XrDn, X-ray density.</p></caption><graphic xlink:href="ccide-6-071Fig11"></graphic></fig></floats-group></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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